Modern metods for laboratory detection of lupus anticoagulant in patients with antiphospholipid syndrome | Ukrainian Medical Journal

Krasivska V.V.,Stasyshyn O.V.

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Modern metods for laboratory detection of lupus anticoagulant in patients with antiphospholipid syndrome

Modern metods for laboratory detection of lupus anticoagulant in patients with antiphospholipid syndrome

February 27, 2018

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Aim — to research the informational content for antiphospholipid syndrome (APS) diagnostics of routine coagulogical and modern methods of lupus anticoagulant (LA) detection in patients with thrombosis and pregnancy complications. Object and methods. Routine coagulogical research and аntiphospholipid antibodies identification, including LA, were carried out in 233 patients with the suspicion of APS with thrombosis in different locations and obstetrical pathologies. Results. According to the international criteria of diagnostics 44.6% patients were diagnosed with APS, among which primary APS — in 85.6% and secondary — in 14.4%. LA was identified in 42.5% patients, which is 95.2% of all patients with APS. While carrying out the routine research of haemostasis system, LA can be detected with the help of prolonged prothrombin time and activated partial thromboplastin time (APTT). On the I screening stage of LA detection the most detailed informational value appears while combining the tests based on differential principals and responsible for various parts of haemostasis, in particular the clotting time with phospholipid dependent diluted viper venom time, APTT with LA-sensitive reagent and prothrombin time with diluted to 50 and 500 times thromboplastin. Conclusions. The correctional tests results are not dependable and do not allow to make a decision as to continuation or termination of the further research. The necessity of their execution should be considered individually. Due to the high informational value, confirmation tests should be carried out on the second stage of LA detection. The obtained results are according to the latest 2014 guidelines, which can be used as standards in LA activity research.

V.V. Кrasivska, O.V. Stasyshyn

Key words: lupus anticoagulant, antiphospholipid antibodies, antiphospholipid syndrome, diagnosis.

Published: 06.03.2018

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